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Table 5 Sensitivity for the ASFV LFIA and qPCR in blood samples post ASFV challenge

From: Development of a chromatographic lateral flow immunoassay for detection of African swine fever virus antigen in blood

Challenge Strain

N

LFIA

qPCR

Pos.

Neg.

Suspect

Sen.

Pos.

Neg.

Sen.

Post-Arm07

56

31

24

1

55.4%

51

5

91.1%

 CS = 0

13

2

11

0

15.4%

9

4

69.2%

 CS = 1–6

29

16

13

0

55.2%

28

1

96.6%

 CS > 6

11

10

0

1

90.9%

11

0

100.0%

 Dead

3

3

0

0

100.0%

3

0

100.0%

 F = 0

20

5

14

1

25.0%

15

5

75.0%

 F = 1

7

2

5

0

28.6%

7

0

100.0%

 F = 2

8

5

3

0

62.5%

8

0

100.0%

 F = 3

15

13

2

0

86.7%

15

0

100.0%

 F = 4

3

3

0

0

100.0%

3

0

100.0%

Post-E70

8

6

2

0

75.0%

8

0

100.0%

 CS = 0

3

2

1

0

66.7%

3

0

100.0%

 CS = 1–6

3

2

1

0

66.7%

3

0

100.0%

 CS > 6

2

2

0

0

100.0%

2

0

100.0%

 Dead

0

 F = 0

4

2

2

0

50.0%

4

0

100.0%

 F = 1

1

1

0

0

100.0%

1

0

100.0%

 F = 2

0

 F = 3

3

3

0

0

100.0%

3

0

100.0%

 F = 4

0

Post-Ken05/Tk1

10

0

10

0

0.0%

10

0

100.0%

  1. CS clinical score, F fever score, Pos. positive result, Neg. negative result, Sen. sensitivity