Fig. 4

The detection of ASFV p30 expressions by immunofluorescence assay. The PAMs of domestic pigs grown in 24-well plates were infected with three ASFVs (P5) at a MOI (multiplicity of infection) of 0.1. At 48 h post infections, the cells were fixed, permeabilized, sequentially probed with anti-p30 mAb and goat anti-mouse IgG H&L Alexa Fluor 594, and counterstained with nucleus marker DAPI. The stained cells were examined for fluorescence signals by fluorescence microscope. The scale bar is 100 μM