Table 1 Primers used in this study
Name | Sequence (5' to 3') | Product length (bp) |
|---|---|---|
ASC | F: TTACCGGACAGCAGCCAAG | 124 |
R: CTCTGACAGGACTTTCCCATAC | ||
Bax | F: GAGTCCAGGCACCTCTTCCC | 107 |
R: CTGCTCGATCTTGGATGAAACCC | ||
BCL2 | F: GAACTGTACGGCCCCACCATGC | 89 |
R: CAAGCTCCCACCAGGGCCAGA | ||
ZO-1 | F: AGCAGAAGCCTCATCTCCAGT | 113 |
R: TAGGCCCCTCCAGTCTGACA | ||
ZO-2 | F: CTCAACCTAAAGCAGCCCCAA | 143 |
R: TATCCCAACGTCATTGCCACCA | ||
Occludin | F: CCTTTTGTTTTATCGCTGCAT | 105 |
R: AGGCACTCAGTATTATTACAGT | ||
TLR4 | F: TTCTCTAACATGCCAAACCTG | 96 |
R: TGGCATTTTATGTAGAACCTG | ||
Myd88 | F: TACTGCCCCAGCGATATCCA | 80 |
R: ACACACAACTTCAGCCGAT | ||
p65 | F: AGCTCCCCAGTCCTATCCCT | 87 |
R: ATCTGCCCCGAAGAAAAGACCA | ||
GAPDH | F: ATATTGTCGCCATCAATGACCC | 116 |
R: AAGTTTCCCGTTCTCAGCCTT | ||
TNF-α | F: CGACGTGCCAATGCCCTCC | 115 |
R: ATCCTTGGCCCTTGAAGAGGAC | ||
IL-6 | F: GTGTGAAGACAGCAAGGAGG | 93 |
R: TGATTGAACCCAGATTGGAAGC | ||
IL-1β | F: GGATGGAAAGCCCACCCTAC | 204 |
R: TCCTGGCCACCTCTGGTATT | ||
NLRP3 | F: AGTGCTGCCTTTCCTATCGG | 103 |
R: AAGTCTCCCAGGGCGTTG | ||
Caspase-1 | F: TTAATGTCTCACGGCATCCTG | 114 |
R: GAGGCAGTGACGGTTGTTG | ||
Caspase-3 | F: AAAATGATCTCACATGCGAAG | 120 |
R: AAATTATTCCTTCATCCCCAT | ||
Caspase-9 | F: CTGGATGCCGTGTCTAGTTTG | 97 |
R: AGCCGCTCTTGGGATTTC | ||
RIPK1 | F: ATGGACAGGCAGACGAAACC | 92 |
R: TGTGCAAAAGGGTCATGGGAG | ||
RIPK3 | F: TTGCCTGATGTAAACCGAAAGG | 111 |
R: CGATGTCTGGGCCACTATCTC | ||
Claudin-1 | F: AGTGTATGAAGTGCATGGAAGAC | 98 |
R: AACTAAAACAGCCAGACCTGC | ||
E-cadherin | F: ACCAGGTTTGGAACGGGAC | 92 |
R: GGCGTTTGGATCATCAGCATC |