Fig. 4
Effects of different concentrations of TDCA on diquat-induced oxidative stress and barrier function in intestinal epithelial cells in vitro. A Experimental grouping scheme. B-F MDA, SOD, GSH-Px, CAT, and T-AOC contents of MODE-K cells in each group. G Nrf2, ZO-1, and Occludin protein expression was analyzed via western blotting in the CON, DQ, and DQ + TDCA (50) groups. H-J Expression of the Nrf2, ZO-1 and Occludin proteins in each group. n = 3 for B-J. All the data are presented as the means ± SEMs. When the DQ group was compared with the CON group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; when the DQ + TDCA group was compared with the DQ group, #P < 0.05, ##P < 0.01, ####P < 0.0001. CON, control; DQ, diquat; TDCA, taurodeoxycholic acid; MDA, malondialdehyde; SOD, superoxide dismutase; GSH-Px, glutathione peroxidase; CAT, catalase; T-AOC, total antioxidant capacity; Nrf2, nuclear factor erythroid 2-related factor 2; TDCA (10) indicates treatment with 10 μM TDCA; TDCA (30) indicates treatment with 30 μM TDCA; and TDCA (50) indicates treatment with 50 μM TDCA