Fig. 4

Muscle-specific knockout of G9a improves NMJ-associated gene expression in aged mice. A KEGG pathway analysis of the 15 pathways enriched in the RNA sequencing data of TAs from aged WT and Ehmt2^Ckmm-KO mice. B-C Differentially expressed genes associated with synapse formation in the TA of aged WT and Ehmt2^Ckmm-KO mice are shown as heatmaps (B) and qPCR results (C). D Gene Ontology (GO) enrichment analysis of gene transcripts in the TAs of aged WT and Ehmt2^Ckmm-KO mice. E qPCR results of AChR subunits. AChR, acetylcholine receptor; TA, tibialis anterior; n = 5–8 per group; ns, no significant difference; *P < 0.05, **P < 0.01