Fig. 5

Muscle-specific knockout of G9a improves NMJ maintenance in aged mice. A-B Heatmap (A) and qPCR results (B) of genes involved in neuromuscular junction formation and maintenance in the TA of aged WT and Ehmt2^Ckmm KO mice. C Immunofluorescence staining of NMJs in the TAs of aged WT and Ehmt2^Ckmm-KO mice. D Percentages of innervated myofibers in the TAs of aged WT and Ehmt2^Ckmm-KO mice. Syn, Synaptophysin (green); Nef, Neurofilament (green); α-BTX, α-Bungarotoxin (red); TA, tibialis anterior; n = 3–5 per group; ns, no significant difference, *P < 0.05, **P < 0.01