Fig. 6

Muscle-specific knockout of G9a mitigates denervation-induced muscular atrophy. A Representative H&E staining (left) showing the distribution of myofiber CSA (right) in the TAs of sham-operated or denervated WT and Ehmt2^Ckmm KO mice. B qPCR results of Ehmt2, Trim63 and Fbxo32 in the TA of sham-operated or denervated WT and Ehmt2^Ckmm KO mice. C Western blots (top) and quantitative results (bottom) of G9a, MuRF1 and Atrogin-1 expression in the TAs of sham-operated or denervated WT and Ehmt2^Ckmm-KO mice. TA, tibialis anterior; n = 3 per group; (A): WT + Sham vs WT + Denervated, ^&P < 0.05, ^&&P < 0.01; Ehmt2^Ckmm + Sham vs Ehmt2^Ckmm + Denervated, *P < 0.05, **P < 0.01; WT + Denervated vs Ehmt2^Ckmm + Denervated,.^#P < 0.05; (B‒C): ns, no significant difference, *P < 0.05, **P < 0.01