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Fig. 5 | Animal Diseases

Fig. 5

From: Characterization of two neutralizing monoclonal antibodies with conformational epitopes against porcine deltacoronavirus

Fig. 5

Identification of conformational epitopes recognized by the mAbs. (A, B) Western blot analyzed mAb binding to denatured S protein, with anti-PDCoV-S antibody as control. Purified PDCoV S1 proteins (lane 1), PDCoV-infected LLC-PK1 WCL (lane 2), uninfected LLC-PK1 WCL (lane 3). Samples boiled for 5 min (A) or 25 min (B) before loading. (C) 96-well plates coated with native PDCoV S1 (dark bars) and heat-denatured S1 (light bars). Incubated with 1:4000 diluted mAbs, negative mouse serum as control. Absorbance measured at 450 nm

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Animal Diseases

ISSN: 2731-0442