Fig. 3
Baicalein significantly restricts PEDV infection. Vero cells were incubated with baicalein (5,10 μM) and PEDV (MOI = 0.1) as described in the Methods section. A The viability of baicalein-treated cells was estimated via a CCK-8 assay. B Culture lysates were collected for virus titration. The results are expressed as the TCID50. C The effect of baicalein on the inactivation of PEDV proliferation was assessed via a TCID50 assay. D EC50 values were calculated via GraphPad Prism 8.0. E The effects of baicalein on PEDV N gene expression were detected via qRT‒PCR. F The effect of baicalein on the internalization phase of PEDV proliferation was assessed via a TCID50 assay. The data are expressed as the means and standard deviations from three independent experiments and were analyzed via one-way ANOVA. **, 0.001 ≤ p < 0.01; ***, p < 0.001